EFEITOS DA ESCULETINA EM CÉLULAS MESANGIAIS IMORTALIZADAS DE CAMUNDONGOS SUBMETIDAS A MEIO COM ALTA CONCENTRAÇÃO DE GLICOSE
DOI:
https://doi.org/10.56238/arev8n4-078Palavras-chave:
Esculetina, Células Mesangiais, Diabetes Mellitus, Estresse Oxidativo, Estresse NitrosativoResumo
Introdução: O diabetes mellitus (DM) tem sido um importante foco de pesquisa devido à sua alta prevalência e mortalidade no Brasil e no mundo. Caracterizado pela hiperglicemia, o DM leva a comorbidades como aterosclerose, retinopatia, neuropatia e nefropatia. Sua fisiopatologia envolve alterações no funcionamento de enzimas e receptores, inflamação e aumento das espécies reativas de oxigênio (ERO). A esculetina, um polifenol derivado da cumarina, tem demonstrado potente atividade antioxidante, melhorando o perfil lipídico e reduzindo a síntese de citocinas pró-inflamatórias.
Objetivo: Estudar o efeito da esculetina em células mesangiais imortalizadas de camundongos submetidas a meio com alta concentração de glicose.
Métodos: Células mesangiais imortalizadas de camundongos (MiMCs) foram cultivadas em placas de cultura contendo meio DMEM/F12 suplementado com 5% de soro fetal bovino. Posteriormente, as células foram distribuídas em quatro grupos: NG (controle, D-glicose 6,7 mM); NG+ESCt (controle tratado com esculetina hidratada 10, 25, 50, 100 ou 200 μg/mL); HG (alta glicose, D-glicose 30 mM); e HG+ESCt (alta glicose tratada com esculetina hidratada 10, 25, 50, 100 ou 200 μg/mL) por 24, 48 ou 72 horas. Nesses grupos, foram avaliadas a viabilidade celular, a proliferação celular e a capacidade antioxidante da esculetina, além de seus efeitos sobre o estresse oxidativo e nitrosativo. As análises estatísticas foram realizadas com o software GraphPad Prism 9.0. Os resultados são apresentados como média ± erro padrão (EP), sendo P < 0,05 considerado estatisticamente significativo.
Resultados: O teste DPPH revelou o efeito antioxidante da esculetina. A produção de ERO foi maior no grupo HG em comparação ao grupo NG, e a produção de TBARS foi menor nos grupos tratados em relação aos grupos controle. Observou-se aumento da enzima catalase no grupo HG em comparação ao grupo NG, redução nos grupos tratados em relação aos controles e aumento da atividade das enzimas SOD e GPx nos grupos ESCt.
Conclusão: Nossos dados sugerem que a esculetina reduz o estresse oxidativo neste modelo experimental. Estudos futuros podem ser promissores para identificar a esculetina como uma intervenção eficaz na prevenção e no tratamento do DM.
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