ANÁLISIS METAGENÓMICO DE BACTERIAS PRODUCTORAS DE LA ENZIMA ADENILATOCICLASA (EC 4.6.1.1) BAJO DIFERENTES MANEJOS DE SUELO Y BOSQUE
DOI:
https://doi.org/10.56238/arev7n8-204Palabras clave:
Genómica Ambiental, Ecología Microbiana, Enlaces, MicrobiologíaResumen
La comunidad microbiana del suelo desempeña un papel esencial en los procesos ecológicos, como el ciclo de nutrientes y la salud de las plantas, y está regulada por mecanismos moleculares como la señalización a través de la adenilato ciclasa (E.C. 4.6.1.1). Esta enzima es un componente clave de la vía del AMPc, con relevancia funcional en varios contextos microbianos. El objetivo de este estudio fue cuantificar e identificar taxonómicamente las bacterias productoras de adenilato ciclasa en suelos bajo diferentes manejos agrícolas y en áreas de bosque nativo en la región de Dourados - MS, Brasil. Para este fin, se analizaron cinco muestras de suelo mediante secuenciación metagenómica y herramientas bioinformáticas, incluyendo ensamblaje de contig, predicción de ORF, alineamiento de bases de datos locales, análisis taxonómicos y estadísticos. Se identificaron 18,139 secuencias asociadas con la enzima diana, con mayor abundancia en suelos de pastoreo continuo e integración cultivo-ganadería. Los filos Actinobacteria y Proteobacteria fueron predominantes, destacando los géneros Sinorhizobium, Micromonospora y Bifidobacterium, con variaciones significativas entre los manejos. El análisis mostró que las prácticas de manejo afectan directamente la composición y diversidad de las bacterias productoras de la enzima adenilato ciclasa.
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