EFFICACY OZONIZED VEGETABLE OILS IN THE INACTIVATION OF CANDIDA ALBICANS
DOI:
https://doi.org/10.56238/arev7n9-323Keywords:
Candida, Antifungals, Medicinal Plants, OzoneAbstract
Ozonized oils have antifungal, antibacterial and antiviral effects. In this context, the objective of this research was to evaluate the effectiveness of ozonazed vegetable oils in the in vitro inactivation of Candida albicans ATCC 25923. To analyze the antifungal activity, vegetable oils of coconut, palm oil, sunflower, olive and pequi, all fresh and ozonized in concentrations ranging from 0.32% to 100%. The minimum inhibitory concentration was determined by the plate microdilution method, and then the minimum fungicidal concentration and the fungicidal kinetics of the oils were verified. The results showed that the vegetable oils of sunflower, coconut, palm oil, pequi and olive, in natura and ozonized, inactivated Candida albicans. However the in natura oils of palm, sunflower and coconut presented minimum inhibitory and fungicidal concentrations higher than those of ozonized oils, while those of olive and pequi did not show differences. Regarding the ability to reduce colony-forming units as a function of time, it was found that olive and pequi oils, ozonated and in natura, were more effective. Based on the results, the vegetable oils indicate the possibility of using them in the therapy of Candida spp.
Downloads
References
ÁLVARES, C. A.; SVIDZINSKI, T. I. E.; CONSOLARO, M. E. L. Vulvovaginal candidiasis: susceptibility factors of the host and virulence of the yeasts. Jornal Brasileiro de Patologia e Medicina Laboratorial, v. 43, p. 319-327, 2007. DOI: https://doi.org/10.1590/S1676-24442007000500004
AIEMSAARD, J. et al. Antifungal activities of essential oils of Syzygium aromaticum, Piper betle, and Ocimum sanctum against clinical isolates of canine dermatophytes. Sci. Asia, v. 43, p. 223, 2017. DOI: https://doi.org/10.2306/scienceasia1513-1874.2017.43.223
BELTRAN, M. S. et al. Antibacterial activity of ozonized olive (olea europaea l.) And venadillo (swietenia humilis zucc.) Oils against Escherichia coli and Staphylococcus aureus. Journal of Microbiology, Biotechnology and Food Sciences, v. 2021, p. 947-949, 2021.
Bocci V. Scentific and medical aspects of ozone therapy. State of the Art. Arc Med Res 2006; 37:425-35. DOI: https://doi.org/10.1016/j.arcmed.2005.08.006
BOCCI V, ZANARDI I, TRAVAGLI V. Ozonization of human HIV-infected plasmas for producing a global vaccine. Virulence. 2010; 1:215-217. DOI: https://doi.org/10.4161/viru.1.3.11830
CABRAL, I. L. et al. AVALIAÇÃO DA EFICIÊNCIA DO ÓLEO OZONIZADO COMO COMPLEMENTO À TERAPIA MEDICAMENTOSA EM INFECÇÕES CUTÂNEAS CAUSADAS PELA LEISHMANIOSE. 2022.
CARDOSO, I. C. C. et al. Potencial antimicrobiano de óleos vegetais ozonizados frente a espécies bacterianas: uma revisão integrativa. Research, Society and Development, v. 10, n. 2, p. e22410212451-e22410212451, 2021. DOI: https://doi.org/10.33448/rsd-v10i2.12451
CELENZA, G. et al. Antimycotic Activity of Ozonized Oil in Liposome Eye Drops against Candida spp. Translational vision science & technology, v. 9, n. 8, p. 4-4, 2020. DOI: https://doi.org/10.1167/tvst.9.8.4
CLINICAL LABORATORY STANDARDS INSTITUTE (CLSI). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M07-A10. Pennsylvania, United States of America: National Committee for Clinical Laboratory Standards, 2015.
CONTRUCCI, B. A. et al. Avaliação in vitro da suscetibilidade de bactérias gram positivas e negativas quando expostas a óleos vegetais ozonizados in vitro evaluation of the susceptibility of gram positive and negative bacteria when exposed to ozonized plant oils. Brazilian Journal of Health Review, v. 4, n. 5, p. 19662-19672, 2021. DOI: https://doi.org/10.34119/bjhrv4n5-097
GUIMARÃES, N. M. et al. Eficácia de óleos vegetais in natura e ozonizados no controle de Sporothrix schenckii. Int. J. Dev. Res., v.10, p. 41970-41974, 2020.
JEFFERY-SMITH, A. et al. Candida auris: a review of the literature. Clinical microbiology reviews, v. 31, n. 1, p. e00029-17, 2018. DOI: https://doi.org/10.1128/CMR.00029-17
KUME, J. E. P. et al. Uso de óleos essenciais in natura e ozonizados no controle in vitro de Trichophyton mentagrophytes. Research, Society and Development, v. 10, n. 1, p. e4710111233-e4710111233, 2021. DOI: https://doi.org/10.33448/rsd-v10i1.11233
LARKIN, E. et al. The emerging pathogen Candida auris: growth phenotype, virulence factors, activity of antifungals, and effect of SCY-078, a novel glucan synthesis inhibitor, on growth morphology and biofilm formation. Antimicrobial agents and chemotherapy, v. 61, n. 5, p. e02396-16, 2017. DOI: https://doi.org/10.1128/AAC.02396-16
MARTÍNEZ-SANCHEZ, G. Scientific rational for the medical application of ozonized oils, an up-date. Ozone Therapy Global Journal, 2021, 11.1: 239-272.
RENSI, A. M; et al. Avaliação do efeito de óleos ozonizados de girassol e coco no controle propeonibacteriumacnes, XXIV. Congresso brasileiro de engenharia Biomédica. São Jose dos Campos. 2014.
SARDI, J. C. O. et al. Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. Journal of medical microbiology, v. 62, n. 1, p. 10-24, 2013. DOI: https://doi.org/10.1099/jmm.0.045054-0
SILVA, W. R da. Composição química e atividade antimicrobiana de óleos vegetais in natura e ozonizados em Acinetobacter baumannii. 2021.
SYLVESTER, P. W. Optimization of the tetrazolium dye (MTT) colorimetric assay for cellular growth and viability. In: Drug Design and Discovery. Humana Press, 2011. p. 157-168. DOI: https://doi.org/10.1007/978-1-61779-012-6_9
TARA, F. et al. The effects of ozonated olive oil and Clotrimazole cream for treatment of vulvovaginal candidiasis.
WANG, X. et al. The first isolate of Candida auris in China: clinical and biological aspects. Emerging microbes & infections, v. 7, n. 1, p. 1-9, 2018. DOI: https://doi.org/10.1038/s41426-018-0095-0
