SELECCIÓN DE AISLADOS BACTERIANOS PRODUCTORES DE ALFA-AMILASA DE ECOSISTEMAS ACUÁTICOS AMAZÓNICOS
DOI:
https://doi.org/10.56238/levv16n55-046Palabras clave:
Biodiversidad, Microorganismos, Biomoléculas, EnzimaResumen
La biodiversidad amazónica representa una fuente significativa de biomoléculas con potencial para aplicación biotecnológica. Esta biodiversidad puede aprovecharse para el desarrollo de insumos aplicables al sector industrial. En este contexto, las bacterias poseen la capacidad de secretar enzimas que pueden servir para diversos propósitos, y la prospección enzimática se considera fundamental para el desarrollo socioeconómico de Brasil. Ante esto, el objetivo de este proyecto fue prospectar alfa-amilasa a partir de la microbiota bacteriana de un ecosistema acuático amazónico. Para ello, se recolectaron muestras de agua del Lago Mamiá, en la región del medio Solimões (Coari-AM), entre 2022 y 2023. Posteriormente, se identificaron aislados bacterianos con capacidad de hidrolizar almidón. Luego, se extrajo el ADN total de las bacterias amilolíticas para la amplificación de los genes del ARN ribosomal 16S. Además, se midieron las condiciones fisicoquímicas (Temperatura, pH y Conductividad Eléctrica) del ecosistema acuático, junto con análisis cualitativos y cuantitativos de la actividad de alfa-amilasa. En conclusión, la prospección de enzimas de la microbiota bacteriana del ecosistema acuático amazónico es de fundamental importancia para la búsqueda de nuevas biomoléculas que satisfagan las necesidades industriales en bioprocesos, así como para el potencial de aplicación de la enzima en la producción de etanol a partir del procesamiento y fermentación del almidón de yuca cultivado en la región amazónica.
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