SELECTION OF ALPHA-AMYLASE PRODUCING BACTERIAL ISOLATES FROM AMAZONIAN AQUATIC ECOSYSTEM
DOI:
https://doi.org/10.56238/levv16n55-046Keywords:
Biodiversity, Microorganisms, Biomolecules, EnzymeAbstract
The Amazonian biodiversity represents a significant source of biomolecules with potential for biotechnological application. This biodiversity can be harnessed for the development of inputs applicable to the industrial sector. In this context, bacteria possess the ability to secrete enzymes that can serve various purposes, and enzyme prospecting is considered fundamental for Brazil's socioeconomic development. Given this, the objective of this project was to prospect for alpha-amylase from the bacterial microbiota of an Amazonian aquatic ecosystem. To achieve this, water samples were collected from Lake Mamiá in the mid-Solimões region (Coari-AM) between 2022 and 2023. Subsequently, bacterial isolates with the capacity to hydrolyze starch were identified. Total DNA was then extracted from the amylolytic bacteria for amplification of the 16S Ribosomal RNA genes. Furthermore, the physicochemical conditions (Temperature, pH, and Electrical Conductivity) of the aquatic ecosystem were measured, alongside qualitative and quantitative analyses of alpha-amylase activity. In conclusion, the prospecting of enzymes from the bacterial microbiota of the Amazonian aquatic ecosystem is of fundamental importance for the search for novel biomolecules that meet industrial needs in bioprocesses, as well as for the enzyme's potential application in ethanol production from the processing and fermentation of cassava starch cultivated in the Amazon region.
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