ANÁLISE HISTOLÓGICA DA MIOTOXICIDADE DO VENENO DE MICRURUS SPIXII (COBRA CORAL AMAZÔNICA) EM MÚSCULO BIVENTER CERVICIS DE PINTAINHO E SUA NEUTRALIZAÇÃO POR SORO ANTIELAPÍDICO
DOI:
https://doi.org/10.56238/arev8n2-068Palavras-chave:
Biventer cervicis, Microscopia de Luz, Micrurus spixii, Soro Antielapídico, Veneno de SerpenteResumo
Os venenos de cobras corais (Micrurus spp.) são neurotóxicos e miotóxicos. Microscopia de luz foi utilizada para avaliar a miotoxicidade do veneno de Micrurus spixii e o potencial de neutralização do dano celular pelo soro antielapídico. Foram estudados músculos provenientes de preparações de biventer cervicis de pintainho estimulados indiretamente durante 2 h, expostas a (n=3 cada) solução nutritiva de Krebs (controle negativo), veneno (10 µg/mL) e pré-incubação (30 min, 37 oC) de veneno (10 µg/mL) com 0,03 mL de soro antielapídico. Os músculos fixados em formalina 10% e mantidos em álcool 70% foram processados e emblocados em parafina para análise histológica. Cortes de 4 μm foram corados com hematoxilina-eosina e analisados em duplo-cego para verificar a presença de edema, vacúolos, condensação de miofibrilas e células ghost. A incubação com veneno reduziu drasticamente a proporção de células saudáveis, de 66,0 ± 0,1% no grupo controle para 0,92 ± 0,08% no grupo veneno (p<0,05). A pré-incubação do veneno com soro antielapídico protegeu parcialmente contra esta redução (4,8 ± 0,42% de células saudáveis; p<0,05 comparado ao veneno). O veneno aumentou a proporção de células com: edema, condensação de miofibrilas e células ghost, e dobrou a proporção de células vacuoladas. As alterações diminuíram em graus variados após pré-incubação com soro antielapídico. Os resultados mostram que o veneno causou alterações histomorfológicas em músculo biventer cervicis e que o soro antielapídico protegeu significativamente, em diferentes níveis, contra o dano celular na proporção testada de soro antielapídico:veneno.
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