ANALYSIS OF PHYSICOCHEMICAL PROPERTIES OF AH PLUS CEMENT ASSOCIATED WITH ANTIMICROBIAL SUBSTANCES

Abstract

HA Plus is the endodontic cement considered the gold standard, which will perform the function of the union between the gutta-percha interfaces and dentin walls, however, its action against microorganisms is limited. Ambroxol (ABX) is a VII metabolite of bromhexine and has known pharmacological mucokinetic and expectorant actions. N-acetylcysteine is a potent antioxidant derived from cysteine, being a non-antibiotic compound, but with antimicrobial action. Thus, the objective of the present study is to evaluate HA Plus associated with 5% N-acetylcysteine and HA Plus associated with 5% Ambroxol, regarding physical properties (radiopacity, setting time, flow, solubility, compressive strength) and antimicrobial activity. 1 - To evaluate radiopacity, we will use metal rings in which the experimental cements will be inserted and maintained,  these will be X-rayed and processed manually, to be later digitized and analyzed in the Digora 1.51 program. 2 – Setting time: the experimental cements poured into metal rings will be subjected to marking with vertical pressure, using Gilmore needles. 3 – Flow: the cement will be spatulated in the center of a glass plate where another will be placed on it,  both weighing 100g, and, after 10 minutes from the beginning of spatulation, the weight will be removed and the larger and smaller diameter of the cement will be measured with the help of a digital caliper, and the average of the two diameters will be considered the cement flow. 4 – Solubility: 30 acrylic teeth will be filled with the experimental cements. The analyses will be made by comparing the volume in mm³ by the images obtained in micro-computed tomography (micro-CT). Antimicrobial action: under the metal rings filled with the test cements and control group will be induced in vitro biofilm of Enterococcus faecalis. After the 21-day period, the samples will be stained Live/Dead Technique-In Vitro Gen, and then evaluated under the Confocal Laser Scanning Microscope. Under the cement blocks, the adhesion of Ent. faecalis will be analyzed. The results will be submitted to the D'Agostino and Pearson tests for verification of normal distribution.