COMPARATIVE ANALYSIS OF MACROSCOPIC VS MICROSCOPIC MEASUREMENT CRITERIA OF INDUCED TOOTH MOVEMENT
DOI:
https://doi.org/10.56238/levv17n56-014Keywords:
Orthodontic Tooth Movement, Periodontal Ligament, Microscopic, Macroscopic, Experimental Animal ModelsAbstract
This study aims to compare microscopic changes in the periodontal ligament (PDL) thickness with macroscopic tooth displacement in a rat model of orthodontic tooth movement. Seventy rats were divided into two experimental groups (GI and GII, N = 30 each) and a control group (GC, N = 10). GI received continuous orthodontic force with a coil spring appliance (50 cN), while GII received interrupted forces, applied in four-day cycles. The experimental groups were randomly subdivided (N = 10 per subgroup), and euthanized on days 8, 16, and 24 post-appliance installation. The distance between the distal face of the last molar and the mesial face of the first molar was measured to assess macroscopic displacement, calculated by subtracting the measurements of the unmoved side. PDL thickness was measured microscopically, and thickness changes (TC) were calculated by subtracting the mean PDL thickness of the control group. Pearson correlation analysis revealed a strong positive correlation between PDL TC and tooth displacement across all time points (r > 0.83). No significant differences in macroscopic displacement were observed between GI and GII at any time point (p > 0.05), however, the PDL TCs were significantly smaller in GI at all time points (p < 0.02), suggesting faster bone formation in this group. Microscopic changes in PDL TC correlated strongly with macroscopic tooth displacement in our rat model. Bone formation was achieved faster in the continuous force group compared to the interrupted force group while the final tooth displacement was similar.
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References
1. Arnett GW, Jelic JS, Kim J, et al. Soft tissue cephalometric analysis: diagnosis and treatment planning of dentofacial deformity. Am J Orthod Dentofacial Orthop 1999;116(3):239–53. DOI: https://doi.org/10.1016/S0889-5406(99)70234-9
2. Ren Y, Maltha JC, Kuijpers-Jagtman AM. The rat as a model for orthodontic tooth movement-a critical review and a proposed solution. Eur J Orthod 2004;26(5):483–90. DOI: https://doi.org/10.1093/ejo/26.5.483
3. Kirschneck C, Proff P, Fanghaenel J, Behr M, Wahlmann U, Roemer P. Differentiated analysis of orthodontic tooth movement in rats with an improved rat model and three- dimensional imaging. Ann Anat 2013;195(6):539–53. DOI: https://doi.org/10.1016/j.aanat.2013.08.003
4. Cuoghi OA, Tondelli PM, Aiello CA, Mendonça MR de, Costa SC da. Importance of periodontal ligament thickness. Braz Oral Res 2013;27(1):76–9. DOI: https://doi.org/10.1590/S1806-83242013000100014
5. Heller IJ, Nanda R. Effect of metabolic alteration of periodontal fibers on orthodontic tooth movement. An experimental study. Am J Orthod 1979;75(3):239–58. DOI: https://doi.org/10.1016/0002-9416(79)90272-0
6. Miura F, Mogi M, Ohura Y, Hamanaka H. The super-elastic property of the Japanese NiTi alloy wire for use in orthodontics. Am J Orthod Dentofacial Orthop 1986;90(1):1–10. DOI: https://doi.org/10.1016/0889-5406(86)90021-1
7. Miura F, Mogi M, Ohura Y, Karibe M. The super-elastic Japanese NiTi alloy wire for use in orthodontics. Part III. Studies on the Japanese NiTi alloy coil springs. Am J Orthod Dentofacial Orthop 1988;94(2):89–96. DOI: https://doi.org/10.1016/0889-5406(88)90356-3
8. Gianelly AA, Bednar J, Dietz VS. Japanese NiTi coils used to move molars distally. Am J Orthod Dentofacial Orthop 1991;99(6):564–6. DOI: https://doi.org/10.1016/S0889-5406(05)81633-6
9. King GJ, Keeling SD, McCoy EA, Ward TH. Measuring dental drift and orthodontic tooth movement in response to various initial forces in adult rats. Am J Orthod Dentofacial Orthop 1991;99(5):456–65. DOI: https://doi.org/10.1016/S0889-5406(05)81579-3
10. Hauber Gameiro G, Nouer DF, Pereira Neto JS, et al. Effects of short- and long-term celecoxib on orthodontic tooth movement. Angle Orthod 2008;78(5):860–5. DOI: https://doi.org/10.2319/100207-474.1
11. Li Y, Jacox LA, Little SH, Ko C-C. Orthodontic tooth movement: The biology and clinical implications. Kaohsiung J Med Sci 2018;34(4):207–14. DOI: https://doi.org/10.1016/j.kjms.2018.01.007
12. Lv T, Kang N, Wang C, Han X, Chen Y, Bai D. Biologic response of rapid tooth movement with periodontal ligament distraction. Am J Orthod Dentofacial Orthop 2009;136(3):401–11. DOI: https://doi.org/10.1016/j.ajodo.2007.09.017
13. Gonçalves A, Mathelié-Guinlet Q, Ramires F, et al. Biological alterations associated with the orthodontic treatment with conventional appliances and aligners: A systematic review of clinical and preclinical evidence. Heliyon 2024;10(12):e32873. DOI: https://doi.org/10.1016/j.heliyon.2024.e32873
14. Graber TM, Vanarsdall Junior RL, Vig KWL. Orthodontics: Current Principles &Techniques. 4th ed. St Louis: Elsevier, 2005. 1232 p.
15. Jeon HH, Teixeira H, Tsai A. Mechanistic Insight into Orthodontic Tooth Movement Based on Animal Studies: A Critical Review. J Clin Med 2021;10(8):1733. DOI: https://doi.org/10.3390/jcm10081733
16. Nakai Y, Praneetpong N, Ono W, Ono N. Mechanisms of Osteoclastogenesis in Orthodontic Tooth Movement and Orthodontically Induced Tooth Root Resorption. J Bone Metab 2023;30(4):297–310. DOI: https://doi.org/10.11005/jbm.2023.30.4.297
