ANALYSIS OF THE ANTIMICROBIAL EFFICACY OF THE COMBINATION OF ALLICIN, CARVACROL, AND THYMOL AGAINST STAPHYLOCOCCUS AUREUS AND CANDIDA ALBICANS
DOI:
https://doi.org/10.56238/levv16n55-101Keywords:
Phytotherapy, Antimicrobial Resistance, Allicin, Carvacrol, ThymolAbstract
The indiscriminate use of antibiotics has accelerated microbial resistance, becoming a global public health challenge. This study analyzed the efficacy of an aqueous extract composed of allicin (derived from Allium sativum) combined with carvacrol and thymol (derived from Origanum vulgare) against Staphylococcus aureus and Candida albicans strains. The primary objective was to analyze the efficacy of the aqueous extract based on allicin, carvacrol, and thymol for the production of a safe, effective phytotherapeutic drug that prevents microbial resistance as a substitutive treatment alternative for infections caused by C. albicans and S. aureus. Methodology included the determination of Minimum Inhibitory Concentration (MIC) and synergism evaluation via checkerboard. Results revealed that the triple association drastically potentiated antimicrobial activity, reducing MIC values to 16 µg/mL (S. aureus) and 32 µg/mL (C. albicans), up to four times lower than isolated compounds. The FICI analysis (< 1.0) confirmed a synergistic/additive effect, suggesting a multi-target mechanism. It is concluded that the proposed formulation holds promising pharmacological potential as a safe alternative treatment for resistant infections.
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References
AL-TAWALBEH, D. et al. Assessment of carvacrol-antibiotic combinations’ antimicrobial activity against methicillin-resistant Staphylococcus aureus. Frontiers in Microbiology, [S.l.] v. 14, Jan. 2024.
ANDERSON, M. et al. The socio-economic drivers and impacts of Antimicrobial Resistance: implications for policy and research. Copenhague: European Observatory on Health Systems and Policies, 2024. (Policy Brief, 64). Disponível em: https://www.ncbi.nlm.nih.gov/books/NBK610054/. Acesso em: 12 dez. 2025.
ANKRI, S.; MIRELMAN, D. Antimicrobial properties of allicin from garlic. Microbes and Infection, Paris, v. 1, n. 2, p. 125-129, Feb. 1999.
ANTIMICROBIAL RESISTANCE COLLABORATORS. Global Burden of Bacterial Antimicrobial Resistance in 2019: a Systematic Analysis. The Lancet, Londres, v. 399, n. 10325, p. 629–655, 12 fev. 2022.
BRASIL. Agência Nacional de Vigilância Sanitária. Formulário de fitoterápicos da Farmacopeia Brasileira. 2. ed. Brasília: Anvisa, 2021. Disponível em: https://www.gov.br/anvisa/pt-br/assuntos/farmacopeia/formulario-fitoterapico/formulario-de-fitoterapicos-da-farmacopeia-brasileira.pdf. Acesso em: 12 dez. 2025.
HOLETZ, F. B. et al. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 7, p. 1027–1031, Out. 2002.
LAMBERT, R. J. W. et al. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied Microbiology, Oxford, v. 91, n. 3, p. 453-462, Sep. 2001.
NAZZARO, F. et al. Essential oils and antifungal activity. Pharmaceuticals, Basel, v. 10, n. 4, art. 86, 2017.
NOSTRO, A. et al. Effects of oregano, carvacrol and thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms. Journal of Medical Microbiology, Londres, v. 56, n. 4, p. 519-523, Apr. 2007.
PAIVA, M. J. M. et al. Pharmacological and toxicological effects of Amaryllidaceae. Brazilian Journal of Biology, São Carlos, v. 83, e277092, 2023.
PETRAKIS, E. et al. A GC-MS and LC-HRMS perspective on the chemotaxonomic investigation of the natural hybrid Origanum lirium and its parents. Phytochemical Analysis, Chichester, v. 34, n. 3, p. 289-300, abr. 2023.
SHARIFZADEH, A. et al. Potential effect of 2-isopropyl-5-methylphenol (thymol) alone and in combination with fluconazole against clinical isolates of Candida albicans. Journal de Mycologie Médicale, Paris, v. 28, n. 2, p. 294–299, jun. 2018.
